Non-destructive tests are techniques used in material and equipment inspection without permanent damage, applied in the stages of manufacture, construction, assembly and maintenance. They are one of the main tools to control and insure the quality of materials and products, with widely applications in oil / petrochemical, chemical, aeronautical, aerospace, steel industry, naval, electromechanical industries. They represent with capacity of providing information about defects, technological characteristics of the material, or monitoring the degradation in service of components, equipment and structures, which allows the use of useful live extensions in service. The objective of this research was to compare the application of different non-destructive techniques in the detection and size estimation of fatigue cracks, nucleated and propagated intentionally, in structural material. For this purpose, cylindrical specimens of 42CrMo4 steel with a V-notch were subjected to cyclic loading with different cycle numbers, which provided different crack sizes. The length of the cracks was monitored by a magnifying glass positioned on the specimen surface. After the crack nucleation and propagation, all specimens were subjected to non-destructive tests making use of Conventional Ultrasound, Phased Array Ultrasound and Digital Radiography, in order to provide crack indications and crack dimensions. After the inspections, the specimens were cooled down in nitrogen and fractured in three point bending to promote opened crack surfaces. In the sequence, the cracks were measured using a magnifying glass, which allowed a comparison between the indicated and measured defects. In this context, all techniques were adequate for crack indications. On the other hands, for short cracks, the Phased Array Ultrasound was the technique that presented the best susceptibility for estimate the discontinuity dimensions, while Digital Radiography proved to be more efficient for longer cracks.